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![Knockdown Validated: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-NBP1-31327-img0037.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0032.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0036.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0026.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0027.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Vimentin protein stained by Vimentin antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin protein stained by Vimentin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0028.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded U37C xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0005.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Analysis of paraffin-embedded RT2 xenograft, using Vimentin antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0033.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0034.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded mouse testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0035.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Sandwich-ELISA-NBP1-31327-img0039.jpg)
![Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:5000.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:5000.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:1000.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0001.jpg)
![Western Blot: Vimentin [p Ser56] Antibody [NBP3-13119] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (NBP3-13119) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-[p-Ser56]-Antibody-Western-Blot-NBP3-13119-img0002.jpg)